Based on the information in the provided URL, the NR1D2 Antibody (PAC05204) is a valuable tool for research related to the NR1D2 protein, a nuclear receptor involved in the regulation of circadian rhythms and metabolism. This polyclonal antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for use in Western blot applications.NR1D2, also known as Rev-erbbeta, plays a crucial role in the molecular clock machinery, regulating gene expression patterns that govern the body's internal clock and metabolic processes. Dysregulation of NR1D2 has been implicated in various health conditions, including metabolic disorders, sleep disorders, and certain types of cancer.
Therefore, studying the function and expression of NR1D2 is essential for gaining insights into these physiological and pathological processes.By using the NR1D2 Antibody (PAC05204), researchers can effectively detect and analyze NR1D2 protein levels in different cell types and tissues, opening up avenues for further exploration into the role of this nuclear receptor in circadian biology, metabolism, and disease. This antibody offers a valuable tool for investigations in chronobiology, metabolism, and related fields, ultimately contributing to the advancement of scientific knowledge and the development of potential therapeutic strategies targeting NR1D2 signaling pathways.
Western Blot. Positive WB detected in: MCF-7 whole cell lysate. All lanes: NR1D2 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 65 kDa. Observed band size: 65 kDa.
Background:
Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARNTL/BMAL1 and CLOCK. Also regulates genes involved in metabolic functions, including lipid metabolism and the inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-[A/G]GGTCA-3' preceded by an A/T-rich 5' sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two nuclegotides (RevDR-2). Acts as a potent competitive repressor of ROR α (RORA) function and also negatively regulates the expression of NR1D1. Regulates lipid and energy homeostasis in the skeletal muscle via repression of genes involved in lipid metabolism and myogenesis including: CD36, FABP3, FABP4, UCP3, SCD1 and MSTN. Regulates hepatic lipid metabolism via the repression of APOC3. Represses gene expression at a distance in macrophages by inhibiting the transcription of enhancer-derived RNAs (eRNAs). In addition to its activity as a repressor, can also act as a transcriptional activator. Acts as a transcriptional activator of the sterol regulatory element-binding protein 1 (SREBF1) and the inflammatory mediator interleukin-6 (IL6) in the skeletal muscle.
Synonyms:
Nuclear receptor subfamily 1 group D member 2 (Orphan nuclear hormone receptor BD73) (Rev-erb alpha-related receptor) (RVR) (Rev-erb-beta) (V-erbA-related protein 1-related) (EAR-1R), NR1D2
