The NQO1 Polyclonal Antibody (CAB1518) is a valuable tool for researchers studying NQO1, a cytoplasmic enzyme involved in antioxidant defense and detoxification processes. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot applications.NQO1, also known as DT-diaphorase or quinone reductase 1, plays a crucial role in protecting cells from oxidative stress and maintaining cellular redox balance. Its aberrant expression has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
Therefore, investigating the expression and activity of NQO1 is essential for understanding its role in disease pathogenesis and identifying potential therapeutic targets.By utilizing the NQO1 Polyclonal Antibody, researchers can accurately detect and analyze NQO1 protein levels in different cell types and tissues, advancing our knowledge of its functions in health and disease. This antibody is a valuable asset for studies in cancer biology, neurology, and pharmacology, providing insights that may lead to the development of novel treatment strategies targeting NQO1-mediated pathways.
Product Name:
NQO1 Rabbit Polyclonal Antibody
SKU:
CAB1518
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human NQO1 (NP_000894.1).
This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method:
Affinity purification
Gene ID:
1728
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using NQO1 Rabbit pAb (CAB1518) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
