The NPRL3 Antibody (PAC060965) is a high-quality antibody specifically designed for research involving the NPR3L protein. NPR3L is a crucial molecule involved in the mTOR signaling pathway, which plays a key role in cell growth, proliferation, and survival. The antibody is raised in rabbits and is highly specific and reactive with human samples, making it an excellent tool for studying NPR3L in Western blot applications.This antibody binds specifically to the NPR3L protein, allowing for accurate detection and analysis in various cell types. Its high sensitivity and specificity make it an ideal choice for researchers studying the mTOR pathway, cancer biology, and cell signaling.
By understanding the role of NPR3L in these processes, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the NPR3L Antibody (PAC060965) is a valuable tool for researchers looking to investigate the function of NPR3L in cellular processes and disease development. Its superior performance and reliability make it a trusted choice for studies in molecular biology, cancer research, and drug discovery.
IHC image of PACO60965 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot. Positive WB detected in: U87 whole cell lysate. All lanes: NPRL3 antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 64 kDa. Observed band size: 64 kDa.
Background:
As a component of the GATOR1 complex functions as an inhibitor of the amino acid, sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid, sensing branch of the TORC1 pathway.
NPRL3: The function of the encoded protein is not known. [provided by RefSeq, Aug 2011]Protein type: Unknown functionChromosomal Location of Human Ortholog: 16p13.3Molecular Function: GTPase activator activityBiological Process: cellular response to amino acid starvation; negative regulation of TOR signaling pathway
UniProt Protein Details:
NCBI Summary:
The function of the encoded protein is not known. [provided by RefSeq, Aug 2011]
