The NPR1 Polyclonal Antibody (CAB10445) is a powerful tool for researchers studying NPR1, a key player in plant defense mechanisms. This antibody, produced in rabbits, exhibits high reactivity with plant samples and has been validated for use in Western blot applications. By binding specifically to the NPR1 protein, this antibody allows for accurate detection and analysis in various plant tissues and cell types, making it an essential resource for studies in plant biology and agricultural research.NPR1, or nonexpresser of PR genes 1, is a crucial regulator of plant immune responses, playing a critical role in the activation of defense genes in plants under stress conditions.
Its involvement in pathogen resistance and systemic acquired resistance makes it a valuable target for research into plant disease resistance and crop protection. Understanding the function of NPR1 is essential for developing sustainable agricultural strategies and enhancing crop resilience in the face of environmental challenges.In summary, the NPR1 Polyclonal Antibody (CAB10445) is a reliable tool for investigating NPR1 function in plants, paving the way for advancements in plant biology, crop protection, and agricultural sustainability.
Product Name:
NPR1 Rabbit Polyclonal Antibody
SKU:
CAB10445
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-470 of human NPR1 (NP_000897.3).
Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).
Purification Method:
Affinity purification
Gene ID:
4881
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NPR1 antibody (CAB10445) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 40s.
