The NPHP4 Polyclonal Antibody (CAB8934) is a valuable tool for researchers studying NPHP4, a protein involved in renal and retinal development. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in immunofluorescence and immunohistochemistry applications. By binding specifically to the NPHP4 protein, this antibody enables the visualization and analysis of NPHP4 expression in a variety of cell and tissue types, making it a versatile tool for investigations in nephrology and ophthalmology research.NPHP4, also known as nephrocystin-4, plays a crucial role in the structure and function of cilia, cellular structures important for cell signaling and sensory functions.
Mutations in the NPHP4 gene are associated with nephronophthisis, a genetic disorder characterized by kidney abnormalities, as well as retinitis pigmentosa, a degenerative eye disease. Research on NPHP4 is essential for understanding the molecular mechanisms underlying these conditions and developing potential therapies for patients affected by these disorders. The NPHP4 Polyclonal Antibody offers researchers a reliable means of exploring the functions and dysregulation of NPHP4 in disease settings, advancing our knowledge of these complex genetic disorders and guiding the development of targeted treatments.
Product Name:
NPHP4 Rabbit Polyclonal Antibody
SKU:
CAB8934
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1247-1426 of human NPHP4 (NP_055917.1).
This gene encodes a protein involved in renal tubular development and function. This protein interacts with nephrocystin, and belongs to a multifunctional complex that is localized to actin- and microtubule-based structures. Mutations in this gene are associated with nephronophthisis type 4, a renal disease, and with Senior-Loken syndrome type 4, a combination of nephronophthisis and retinitis pigmentosa. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
261734
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NPHP4 Rabbit pAb (CAB8934) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
