The NPC1L1 Polyclonal Antibody (CAB10049) is a valuable tool for research involving the NPC1L1 protein, a key player in lipid metabolism and cholesterol absorption. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in a variety of applications, including Western blotting. By binding specifically to the NPC1L1 protein, this antibody enables the detection and analysis of NPC1L1 in different cell types, making it a versatile choice for studies in lipid biology and cardiovascular research.NPC1L1 is a critical transporter involved in the uptake of cholesterol from the diet, making it a potential target for therapeutic interventions aimed at lowering cholesterol levels and reducing the risk of cardiovascular diseases.
Research into NPC1L1 can provide insights into the mechanisms of cholesterol absorption and metabolism, offering new avenues for developing treatments for hypercholesterolemia and related disorders. The NPC1L1 Polyclonal Antibody is a valuable tool for investigators studying lipid transport and cholesterol homeostasis, offering a reliable means of investigating the role of NPC1L1 in health and disease.
Product Name:
NPC1L1 Rabbit Polyclonal Antibody
SKU:
CAB10049
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1030-1100 of human Niemann-Pick type C1 Like-1 (NP_001095118.1).
The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
29881
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using Niemann-Pick type C1 Like-1 Rabbit pAb (CAB10049) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
