The Notch4 Polyclonal Antibody (CAB8303) is a vital tool for researchers studying the Notch4 protein, a key player in cellular signaling pathways and developmental processes. This antibody, produced in rabbits, shows high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the Notch4 protein, this antibody enables accurate detection and analysis in various cell types, making it an essential component for investigations in the fields of cell biology, development, and cancer research.Notch4 is a transmembrane receptor involved in critical cellular functions such as cell differentiation, proliferation, and apoptosis.
Dysregulation of Notch4 signaling has been implicated in various diseases, including cancer, cardiovascular disorders, and developmental abnormalities. Understanding the role of Notch4 in these processes is essential for the development of targeted therapies and interventions aimed at modulating Notch4 activity for therapeutic purposes. With its high specificity and reliability, the Notch4 Polyclonal Antibody (CAB8303) is a valuable tool for researchers seeking to uncover the intricate mechanisms involving Notch4 and its implications for human health and disease.
Product Name:
NOTCH4 Rabbit Polyclonal Antibody
SKU:
CAB8303
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1824-2003 of human NOTCH4 (NP_004548.3).
Cell membrane, Nucleus, Single-pass type I membrane protein.
Calculated MW:
210kDa
Observed MW:
80kDa
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Purification Method:
Affinity purification
Gene ID:
4855
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of Mouse lung, using NOTCH4 Rabbit pAb (CAB8303) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
