The Notch3 Polyclonal Antibody (CAB3115) is a valuable tool for research involving the Notch3 protein, a key regulator of cell differentiation and development. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it an ideal choice for Western blot applications.Notch3 is a transmembrane receptor involved in various cellular processes, including cell fate determination and tissue patterning. Dysregulation of Notch3 signaling has been implicated in numerous diseases, such as cancer, cardiovascular disorders, and neurodegenerative conditions.
Studying the expression and activity of Notch3 using this antibody can provide important insights into its role in these pathological conditions.With its high specificity and reliability, the Notch3 Polyclonal Antibody (CAB3115) is a valuable tool for researchers studying Notch3 biology and its implications in various disease states. By enabling the detection and analysis of Notch3 protein in different cell types, this antibody supports advancements in molecular and cellular biology research.
Product Name:
NOTCH3 Rabbit Polyclonal Antibody
SKU:
CAB3115
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1520-1640 of human NOTCH3 (NP_000426.2).
Cell membrane, Nucleus, Single-pass type I membrane protein.
Calculated MW:
244kDa
Observed MW:
90kDa/280kDa
This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
Purification Method:
Affinity purification
Gene ID:
4854
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using NOTCH3 Rabbit pAb (CAB3115) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
