The Notch3 Polyclonal Antibody (CAB13522) is a valuable tool for researchers investigating the Notch3 protein, a key signaling receptor involved in various cellular processes, including development, differentiation, and disease. Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in Western blot applications.Notch3 is a transmembrane receptor known for its role in controlling cell fate decisions and maintaining tissue homeostasis. Dysregulation of Notch3 signaling has been implicated in several diseases, including cancer, cardiovascular disorders, and neurological conditions.
By specifically binding to Notch3, this polyclonal antibody enables the detection and analysis of Notch3 expression in various cell types, offering insights into its function and potential therapeutic implications.Researchers studying Notch3 biology, signaling pathways, and associated diseases will find the Notch3 Polyclonal Antibody (CAB13522) to be a valuable asset in their research endeavors. Its versatility and specificity make it an essential tool for studies aimed at elucidating the role of Notch3 in health and disease.
Product Name:
NOTCH3 Rabbit Polyclonal Antibody
SKU:
CAB13522
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 2200-2321 of human NOTCH3 (NP_000426.2).
Cell membrane, Nucleus, Single-pass type I membrane protein.
Calculated MW:
244kDa
Observed MW:
90kDa
This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
Purification Method:
Affinity purification
Gene ID:
4854
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from K-562 cells, using NOTCH3 Rabbit pAb (CAB13522) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
