The Notch3 Polyclonal Antibody (CAB0929) is specifically designed for researchers investigating the Notch3 protein, a transmembrane receptor involved in cell signaling and development. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.Notch3 plays a crucial role in various cellular processes, including differentiation, proliferation, and apoptosis. Dysregulation of Notch3 signaling has been implicated in a range of diseases, such as cancer, cardiovascular disorders, and neurological conditions. By targeting the Notch3 protein, researchers can gain valuable insights into the mechanisms driving these diseases and potentially identify novel therapeutic targets.
With its ability to selectively bind to Notch3, this polyclonal antibody enables the detection and analysis of Notch3 expression in different cell types and tissues. Its versatility and reliability make it an essential tool for studies in developmental biology, cancer research, and other fields where Notch3 signaling is of interest. Gain a deeper understanding of Notch3 function and its impact on disease pathogenesis with the Notch3 Polyclonal Antibody (CAB0929).
Product Name:
NOTCH3 Rabbit Polyclonal Antibody
SKU:
CAB0929
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 751-850 of human NOTCH3 (NP_000426.2).
Cell membrane, Nucleus, Single-pass type I membrane protein.
Calculated MW:
244kDa
Observed MW:
Refer to figures
This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
Purification Method:
Affinity purification
Gene ID:
4854
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NOTCH3 antibody (CAB0929) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 15s.
