The Notch2 Polyclonal Antibody (CAB0560) is a valuable tool for researchers studying the Notch2 protein, a critical regulator of cell differentiation and development. This antibody, produced in rabbits, is highly specific to human samples and has been extensively validated for Western blot applications. By binding to the Notch2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in developmental biology, cancer research, and stem cell studies.Notch2 is a key player in various cellular processes, including cell fate determination, proliferation, and apoptosis.
Dysregulation of Notch2 signaling has been linked to numerous diseases, such as cancer, Alzheimer's, and developmental disorders. By understanding the role of Notch2 in these pathological conditions, researchers can uncover potential therapeutic targets and develop strategies to combat these diseases.Overall, the Notch2 Polyclonal Antibody (CAB0560) provides researchers with a reliable tool to unravel the complexities of Notch2 signaling and its impact on cellular behavior, offering valuable insights for potential clinical interventions and treatment strategies.
Product Name:
NOTCH2 Rabbit Polyclonal Antibody
SKU:
CAB0560
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2242-2471 of human NOTCH2 (NP_077719.2).
Cell membrane, Cytoplasm, Nucleus, Single-pass type I membrane protein.
Calculated MW:
265kDa
Observed MW:
120kDa
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4853
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NOTCH2 antibody (CAB0560) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
