The Notch1 Polyclonal Antibody (CAB16673) is a valuable tool for researchers studying Notch1, a critical signaling pathway involved in various cellular processes, including development, differentiation, and proliferation. This antibody is produced in rabbits and is highly specific, allowing for reliable detection of Notch1 protein in human samples. With its validated use in Western blot applications, this antibody enables researchers to study the expression and activity of Notch1 in different cell types and tissues.
By targeting Notch1, researchers can gain insights into the mechanisms underlying various diseases, including cancer, developmental disorders, and cardiovascular conditions.Overall, the Notch1 Polyclonal Antibody is essential for advancing our understanding of Notch1 signaling and its role in health and disease. Its high reactivity and specificity make it a valuable tool for immunology, oncology, and developmental biology research.
Product Name:
Notch1 Rabbit Polyclonal Antibody
SKU:
CAB16673
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 2455-2555 of human Notch1 (NP_060087.3).
Cell membrane, Nucleus, Single-pass type I membrane protein.
Calculated MW:
273kDa
Observed MW:
120kDa
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.
Purification Method:
Affinity purification
Gene ID:
4851
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-251MG cells, using Notch1 Rabbit pAb (CAB16673) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 300s.
