The NOS3 Polyclonal Antibody (CAB1548) is a valuable tool for research involving the nitric oxide synthase 3 (NOS3) enzyme, which plays a crucial role in the production of nitric oxide, a key signaling molecule in the cardiovascular system. This antibody, developed through rabbit immunization, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications. By binding to NOS3, this antibody enables the detection and analysis of the enzyme in various cell types, facilitating research in cardiovascular physiology and pathology.
NOS3 is known to regulate blood flow, vascular tone, and platelet function, making it a promising target for studying cardiovascular disorders such as hypertension, atherosclerosis, and heart failure. Understanding the function and regulation of NOS3 is essential for developing novel therapies that target the nitric oxide pathway to improve cardiovascular health. The NOS3 Polyclonal Antibody provides researchers with a reliable tool to investigate the role of NOS3 in disease mechanisms and develop innovative treatments for cardiovascular conditions.
Product Name:
eNOS Rabbit Polyclonal Antibody
SKU:
CAB1548
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1203 of human eNOS (NP_000594.2).
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4846
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using eNOS antibody (CAB1548) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
