Anti-NOS2 Antibody (CAB14031)
- SKU:
- CAB14031
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Anti-NOS2 Antibody (CAB14031)
The NOS2 Polyclonal Antibody (CAB14031) is a valuable tool for researchers studying nitric oxide synthase 2 (NOS2), an enzyme involved in the production of nitric oxide during immune responses. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blot applications.NOS2 plays a crucial role in the regulation of immune responses, particularly in the context of inflammation and host defense against pathogens. By targeting NOS2 with this antibody, researchers can gain valuable insights into the function and regulation of this enzyme in various cell types.
This makes the antibody ideal for studies in immunology, infectious diseases, and inflammatory conditions.The ability to detect and analyze NOS2 using this polyclonal antibody opens up opportunities for understanding the role of nitric oxide in disease processes and developing targeted therapies. By investigating the activity of NOS2, researchers can uncover potential treatment strategies for conditions such as sepsis, autoimmune diseases, and neuroinflammatory disorders.
| Antibody Name: | Anti-NOS2 Antibody |
| Antibody SKU: | CAB14031 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-730 of human NOS2 (NP_000616.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | RAW264.7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-730 of human NOS2 (NP_000616.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VWQD EKRR PKRR EIPL KVLV KAVL FACM LMRK TMAS RVRV TILF ATET GKSE ALAW DLGA LFSC AFNP KVVC MDKY RLSC LEEE RLLL VVTS TFGN GDCP GNGE KLKK SLFM LKEL NNKF RYAV FGLG SSMY PRFC AFAH DIDQ KLSH LGAS QLTP MGEG DELS GQED AFRS WAVQ TFKA ACET FDVR GKQH IQIP KLYT SNVT WDPH HYRL VQDS QPLD LSKA LSSM HAK |
| Gene ID: | 4843 |
| Uniprot: | P35228 |
| Cellular Location: | |
| Calculated MW: | 126kDa/131kDa |
| Observed MW: | 131KDa |
| Synonyms: | NOS, INOS, NOS2A, HEP-NOS, iNOS, NOS2 |
| Background: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. |
| UniProt Protein Function: | Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. |
| UniProt Protein Details: | Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group By similarity.Binds 1 FAD By similarity.Binds 1 FMN By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity. Enzyme regulation: Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity By similarity. Subunit structure: Homodimer. Binds SLC9A3R1. Tissue specificity: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Induction: By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Ref.14 Polymorphism: Note=Genetic variations in NOS2 are involved in resistance to malaria [ MIM:611162]. Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain. |
| NCBI Summary: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35228 |
| NCBI GenInfo Identifier: | 1352513 |
| NCBI Gene ID: | 4843 |
| NCBI Accession: | P35228.2 |
| UniProt Secondary Accession: | P35228,O60757, O94994, Q16263, Q16692, Q4TTS5, Q9UD42 A1L3U5, B7ZLY2, |
| UniProt Related Accession: | P35228 |
| Molecular Weight: | 131,117 Da |
| NCBI Full Name: | Nitric oxide synthase, inducible |
| NCBI Synonym Full Names: | nitric oxide synthase 2, inducible |
| NCBI Official Symbol: | NOS2Â Â |
| NCBI Official Synonym Symbols: | NOS; INOS; NOS2A; HEP-NOSÂ Â |
| NCBI Protein Information: | nitric oxide synthase, inducible; NOS type II; NOS, type II; inducible NOS; hepatocyte NOS; inducible NO synthase; nitric oxide synthase, macrophage; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes) |
| UniProt Protein Name: | Nitric oxide synthase, inducible |
| UniProt Synonym Protein Names: | Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2 |
| Protein Family: | Inositol 3-kinase |
| UniProt Gene Name: | NOS2Â Â |
| UniProt Entry Name: | NOS2_HUMAN |
 | Western blot analysis of extracts of RAW264.7 cells, using NOS2 antibody (CAB14031) at 1:1000 dilution.Raw264.7 cells were treated by LPS (1 ug/ml) at 37'C for over night. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using NOS2 antibody (CAB14031) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using NOS2 antibody (CAB14031) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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