The Noggin Polyclonal Antibody (CAB8305) is a crucial tool for researchers studying the effects of Noggin, a secreted protein that plays a key role in regulating bone formation and development. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in applications such as Western blotting.Noggin is a critical factor in the regulation of bone morphogenetic proteins (BMPs), which are essential for proper skeletal development and maintenance. By binding to BMPs, Noggin acts as an antagonist, blocking their signaling pathways and thereby influencing various cellular processes.
Its role in skeletal development makes Noggin a key target for research in bone disorders, regeneration, and potential therapeutic interventions.The Noggin Polyclonal Antibody enables precise detection and analysis of Noggin protein levels in various cell types, providing valuable insights for studies in bone biology, developmental biology, and regenerative medicine. Understanding the mechanisms by which Noggin interacts with BMPs is crucial for unraveling the complexities of skeletal development and devising novel strategies for treating bone-related conditions.
Product Name:
NOG Rabbit Polyclonal Antibody
SKU:
CAB8305
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 28-232 of human NOG (NP_005441.1).
The secreted polypeptide, encoded by this gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse.
Purification Method:
Affinity purification
Gene ID:
9241
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NOG antibody (CAB8305) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
