The NOB1 Polyclonal Antibody (CAB17690) is a valuable tool for researchers studying the NOB1 protein, a key enzyme involved in ribosome biogenesis and protein synthesis. This rabbit polyclonal antibody has been validated for use in various applications, including Western blot, immunofluorescence, and immunohistochemistry, making it versatile for different experimental needs.The NOB1 antibody specifically targets the NOB1 protein, allowing for accurate detection and analysis in a variety of cell types and tissues. Its high reactivity with human samples ensures reliable results in research focused on understanding the role of NOB1 in cellular processes, such as ribosome assembly and protein degradation.
With its potential implications in cancer, neurodegenerative diseases, and aging, investigating the function of NOB1 is vital for uncovering new therapeutic strategies and targets. By using the NOB1 Polyclonal Antibody, researchers can gain valuable insights into the molecular mechanisms involving NOB1 and its impact on various pathological conditions.
Product Name:
NOB1 Rabbit Polyclonal Antibody
SKU:
CAB17690
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human NOB1 (NP_054781.1).
In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA (Lamanna and Karbstein, 2009 [PubMed 19706509]).
Purification Method:
Affinity purification
Gene ID:
28987
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NOB1 Rabbit pAb (CAB17690) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
