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NLRP3 Rabbit Polyclonal Antibody (CAB5652)

SKU:
CAB5652
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
$167 - $503
Frequently bought together:

Description

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NLRP3 Rabbit Polyclonal Antibody (CAB5652)

The NLRP3 Polyclonal Antibody (CAB5652) is a valuable tool for studying NLRP3, a key player in the regulation of inflammation and immune responses. This antibody is raised in rabbits and is highly specific to human samples, making it ideal for use in Western blot applications. By targeting the NLRP3 protein, researchers can gain insights into its role in various cell types and its implications for diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions.

NLRP3, also known as NOD-like receptor protein 3, is a critical component of the innate immune system, playing a crucial role in the activation of inflammatory responses. Dysregulation of NLRP3 has been linked to a variety of diseases, making it a promising target for therapeutic interventions. By using the NLRP3 Polyclonal Antibody, researchers can further explore the functions of NLRP3 and potentially uncover new treatment strategies for immune-related disorders.

Product Name:NLRP3 Rabbit Polyclonal Antibody
SKU:CAB5652
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of mouse NLRP3 (NP_665826.1).
Sequence:MTSV RCKL AQYL EDLE DVDL KKFK MHLE DYPP EKGC IPVP RGQM EKAD HLDL ATLM IDFN GEEK AWAM AVWI FAAI NRRD LWEK AKKD QPEW N
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:1000 - 1:5000 IF/ICC,1:50 - 1:200
Synonyms:AII; AVP; FCU; MWS; FCAS; KEFH; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; DFNA34; PYPAF1; AGTAVPRL; NLRP3
Positive Sample:Raji
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Endoplasmic reticulum, Inflammasome, Nucleus, Secreted, cytosol.
Calculated MW:118kDa
Observed MW:85kDa/110kDa

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, and deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.

Purification Method:Affinity purification
Gene ID:114548
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Raji cells using NLRP3 Rabbit pAb(CAB5652) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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