The NLRP3 Polyclonal Antibody (CAB21906) is a valuable tool for researchers studying the NLRP3 protein, an inflammasome component involved in immune responses and inflammation. Produced in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications. By targeting the NLRP3 protein, this antibody allows for the detection and analysis of NLRP3 in various cell types, making it an ideal choice for studies in immunology and inflammatory diseases.
NLRP3, also known as NLR family pyrin domain containing 3, is a key player in the innate immune response, particularly in the activation of the inflammasome complex. Dysregulation of NLRP3 has been linked to various inflammatory disorders, including autoimmune diseases and metabolic disorders. By understanding the function of NLRP3, researchers can gain valuable insights into the mechanisms underlying these conditions and develop potential therapeutic interventions targeting the NLRP3 pathway.
Product Name:
NLRP3 Polyclonal Antibody
SKU:
CAB21906
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of mouse NLRP3 (NP_665826.1).
Enables DNA-binding transcription factor binding activity and sequence-specific DNA binding activity. Involved in several processes, including positive regulation of T-helper cell differentiation; positive regulation of cytokine production; and response to bacterium. Acts upstream of or within several processes, including NLRP3 inflammasome complex assembly; activation of cysteine-type endopeptidase activity involved in apoptotic process; and defense response to virus. Located in cytoplasm and nucleus. Part of NLRP3 inflammasome complex. Is expressed in central nervous system and retina. Used to study CINCA Syndrome; familial cold autoinflammatory syndrome 1; and non-alcoholic fatty liver disease. Human ortholog(s) of this gene implicated in CINCA Syndrome; Muckle-Wells syndrome; autosomal dominant nonsyndromic deafness 34; familial cold autoinflammatory syndrome 1; and urticaria. Orthologous to human NLRP3 (NLR family pyrin domain containing 3).
Purification Method:
Affinity purification
Gene ID:
216799
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from RAW 264.7 cells using NLRP3 Rabbit pAb (CAB21906) at 1:5000 dilution. Raw 264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 1s.
