The NLRP3 Monoclonal Antibody is a valuable tool for researchers studying the NLRP3 inflammasome, an important component of the innate immune system. This antibody, produced in mice, is highly specific to the NLRP3 protein and is validated for use in immunofluorescence and immunohistochemistry applications. By targeting NLRP3, researchers can investigate the role of this protein in inflammatory pathways, making it ideal for studies on inflammatory diseases, autoimmune disorders, and metabolic conditions.
The NLRP3 inflammasome is a key player in the body's response to infection and tissue damage, as well as in the development of chronic inflammatory diseases. Understanding the regulation and activation of NLRP3 is crucial for developing targeted therapies to modulate the immune response and treat conditions such as gout, diabetes, and Alzheimer's disease. The NLRP3 Monoclonal Antibody provides researchers with a reliable tool to explore the complex functions of NLRP3 in health and disease.
Product Name:
NLRP3 Monoclonal Antibody
SKU:
CAB24294
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of mouse NLRP3(NP_665826.1).
Enables DNA-binding transcription factor binding activity and sequence-specific DNA binding activity. Involved in several processes, including positive regulation of T-helper cell differentiation; positive regulation of cytokine production; and response to bacterium. Acts upstream of or within several processes, including NLRP3 inflammasome complex assembly; activation of cysteine-type endopeptidase activity involved in apoptotic process; and defense response to virus. Located in cytoplasm and nucleus. Part of NLRP3 inflammasome complex. Is expressed in central nervous system and retina. Used to study CINCA Syndrome; familial cold autoinflammatory syndrome 1; and non-alcoholic fatty liver disease. Human ortholog(s) of this gene implicated in CINCA Syndrome; Muckle-Wells syndrome; autosomal dominant nonsyndromic deafness 34; familial cold autoinflammatory syndrome 1; and urticaria. Orthologous to human NLRP3 (NLR family pyrin domain containing 3).
Purification Method:
Affinity purification
Gene ID:
216799
Clone Number:
ARC62768
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from RAW 264.7 cells, using NLRP3 Rabbit mAb (CAB24294) at 1:1000 dilution.Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
