Nkx3.2 Polyclonal Antibody (CAB8093) is a specialized tool for researchers investigating the role of Nkx3.2, a transcription factor known for its involvement in skeletal development and cartilage formation. This polyclonal antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it a reliable choice for Western blot analysis.Nkx3.2, also referred to as Bapx1, plays a crucial role in regulating gene expression and controlling cell differentiation during embryonic development. Its expression has been linked to various musculoskeletal disorders, making it a potential target for therapeutic interventions aimed at treating conditions such as osteoarthritis and chondrodysplasia.
By using the Nkx3.2 Polyclonal Antibody, researchers can detect and analyze the expression levels of Nkx3.2 in different cell types, facilitating a better understanding of its functions and mechanisms of action. This antibody is a valuable tool for studies focusing on skeletal biology, developmental biology, and musculoskeletal diseases, providing valuable insights into the molecular pathways regulated by Nkx3.2.
Product Name:
NKX3-2 Rabbit Polyclonal Antibody
SKU:
CAB8093
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human NKX3-2 (NP_001180.1).
Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in cell differentiation; negative regulation of chondrocyte differentiation; and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including animal organ development; embryonic skeletal system development; and intestinal epithelial cell development. Predicted to be part of chromatin. Predicted to be active in nucleus.
Purification Method:
Affinity purification
Gene ID:
579
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with NKX3-2, using NKX3-2 Rabbit pAb (CAB8093) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30 s.
