The NKX2-5 Polyclonal Antibody (CAB5651) is a valuable tool for researchers studying the NKX2-5 protein, which is involved in the development of the heart and plays a critical role in cardiac morphogenesis. This antibody, generated in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications.NKX2-5 is a transcription factor that is essential for proper heart development and function. Mutations in the NKX2-5 gene have been linked to congenital heart defects and other cardiac abnormalities, making it an important target for research in cardiology and developmental biology.
By targeting the NKX2-5 protein, this antibody allows for the detection and analysis of NKX2-5 in various cell types, providing valuable insights into its role in heart development and disease. Its specificity and reliability make it an ideal tool for studies aimed at understanding the mechanisms underlying cardiac development and dysfunction.
Product Name:
NKX2-5 Rabbit Polyclonal Antibody
SKU:
CAB5651
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human NKX2-5 (NP_004378.1).
This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1482
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NKX2-5 antibody (CAB5651) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 60s.
