The NKX2-1 Polyclonal Antibody (CAB3097) is a valuable tool for researchers studying the NKX2-1 protein, a transcription factor involved in lung and thyroid development. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in various applications, including Western blotting.NKX2-1, also referred to as thyroid transcription factor 1 (TTF-1), plays a crucial role in the regulation of genes involved in lung and thyroid differentiation. Dysregulation of NKX2-1 has been implicated in various diseases, including lung cancer and thyroid disorders, making it a key target for research in the fields of oncology and endocrinology.
By utilizing the NKX2-1 Polyclonal Antibody, researchers can analyze the expression and function of NKX2-1 in different cell types, providing valuable insights into its role in disease development and progression. This antibody is essential for advancing our understanding of NKX2-1-mediated pathways and potential therapeutic strategies for related disorders.
Product Name:
NKX2-1 Rabbit Polyclonal Antibody
SKU:
CAB3097
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human NKX2-1 (NP_003308.1).
This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription.
Purification Method:
Affinity purification
Gene ID:
7080
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of NCI-H460 cells, using NKX2-1 antibody (CAB3097) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
