The NKRF Polyclonal Antibody (CAB4853) is a valuable tool for researchers studying the NKRF protein, which plays a crucial role in the regulation of gene expression and immune responses. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications.NKRF, also known as NF-kappa-B-repressing factor, is a transcriptional regulator that modulates the activity of NF-kappa-B, a key player in the immune response. By targeting NKRF, researchers can gain insights into the mechanisms underlying inflammatory processes, autoimmune diseases, and cancer development.
The NKRF Polyclonal Antibody enables the detection and analysis of NKRF protein levels in various cell types, making it a valuable tool for immunology and cancer research. Understanding the role of NKRF in immune regulation may lead to the development of novel therapeutic strategies for treating a range of immune-related disorders.
Product Name:
NKRF Rabbit Polyclonal Antibody
SKU:
CAB4853
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 391-690 of human NKRF (NP_060014.2).
This gene encodes a transcriptional repressor that interacts with specific negative regulatory elements to mediate transcriptional repression of certain nuclear factor kappa B responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
55922
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NKRF antibody (CAB4853) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
