The NIT2 Polyclonal Antibody (PAC018757) is a valuable tool for researchers studying NIT2, a key enzyme involved in the regulation of nitrogen metabolism and cell growth. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications. By binding to the NIT2 protein, this antibody enables researchers to analyze and detect NIT2 expression in a variety of cell types, facilitating investigations in fields such as metabolism, cancer biology, and biomarker discovery.
NIT2, also known as nitrilase homolog 2, plays a crucial role in the detoxification and metabolism of various nitrogenous compounds, contributing to cellular homeostasis and overall health. Dysregulation of NIT2 activity has been implicated in diseases such as metabolic disorders and cancer, making it an attractive target for therapeutic intervention. By elucidating the function of NIT2, researchers can uncover novel avenues for the development of treatments and interventions aimed at improving human health and fighting disease.
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using PACO18757(NIT2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Mouse liver tissue, Mouse kidney tissue, RAW264.7 cells, Primary antibody: PACO18757(NIT2 Antibody) at dilution 1/950, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 90 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO18757(NIT2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.
Synonyms:
Itrilase family, member 2
UniProt Protein Function:
NIT2: a protein of unknown function. A CN hydrolase domain suggests that this protein may be involved in nitrilase-related metabolism and handling.
