The Nicastrin Polyclonal Antibody (CAB0128) is an essential tool for researchers studying Nicastrin, a key component of the gamma-secretase complex involved in the proteolytic processing of various transmembrane proteins, including amyloid precursor protein implicated in Alzheimer's disease. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications.
By targeting Nicastrin, researchers can explore its role in regulating protein processing and its potential involvement in neurodegenerative diseases like Alzheimer's. This antibody is crucial for advancing our understanding of Nicastrin's functions and its potential as a therapeutic target in the treatment of neurodegenerative disorders.
Product Name:
NCSTN Rabbit Polyclonal Antibody
SKU:
CAB0128
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 250-578 of human NCSTN (NP_056146.1).
Melanosome, Membrane, Single-pass type I membrane protein.
Calculated MW:
78kDa
Observed MW:
100-120kDa
This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Purification Method:
Affinity purification
Gene ID:
23385
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of C6, using NCSTN Rabbit pAb (CAB0128) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
