The NHP2 Polyclonal Antibody (CAB5991) is specifically designed for research involving NHP2, a nucleolar protein associated with ribosome biogenesis and telomere maintenance. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. It targets the NHP2 protein, facilitating its detection and analysis in a variety of cell types, making it an excellent tool for studies in molecular biology and oncology research.
NHP2 is a crucial player in maintaining cellular homeostasis by regulating the production of ribosomes and preserving the integrity of telomeres. Dysregulation of NHP2 has been linked to various diseases, including cancer and genetic disorders, making it a promising target for therapeutic intervention. Investigating the function of NHP2 is essential for developing treatments that target these diseases at the molecular level.
Product Name:
NHP2 Rabbit Polyclonal Antibody
SKU:
CAB5991
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human NHP2 (NP_060308.1).
This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nhp2p. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
55651
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NHP2 Rabbit pAb (CAB5991) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
