Description

NHP Cytokine Storm Multiplex Panel 3-plex

Analytes

IL-6, IL-10, and IFN-gamma

Species Reactivity:

Non-Human Primate

Assay Type:

Multiplex

Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

Cytokine Release Syndrome Multiplex Panel 5-plex is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IL-6, IL-10, IFN-gamma on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 3 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Reactivity:	Non-Human Primate
Buffers:	2x dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Analytes:	IL-6, IL-10, and IFN-gamma
Lyophilized Standard Mix:	Lyophilized Standard Mix Non-Human Primate COVID-19 Multiplex Cytokine Immunoassay. One vial containing lyophilized recombinant IL-6, IL-10, IFN-gamma
Sample types:	Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Standard dose recovery:	70-130%
Intra-assay CV:	< 10%
Inter-assay CV:	< 20%
Cross-reactivity of analytes in the Panel:	Negligible
Sample volume:	15 µL/test