The NHLH1 Polyclonal Antibody (CAB15695) is a valuable tool for researchers studying NHLH1, a transcription factor involved in cell differentiation and development. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By targeting the NHLH1 protein, this antibody allows for the detection and analysis of NHLH1 expression in various cell types, making it an ideal choice for studies in developmental biology and gene regulation.
NHLH1, also known as helix-loop-helix protein 1, plays a crucial role in the regulation of gene expression and cell fate determination. Its involvement in various cellular processes makes it a key target for research on developmental disorders, cancer, and neurological diseases. Understanding the function of NHLH1 is essential for unlocking its potential as a therapeutic target and advancing our knowledge of gene regulatory networks.
Product Name:
NHLH1 Rabbit Polyclonal Antibody
SKU:
CAB15695
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human NHLH1 (NP_005589.1).
WB,1:1000 - 1:5000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
HEN1; NSCL; NSCL1; bHLHa35; NHLH1
Positive Sample:
293T transfected with NHLH1
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
15kDa
Observed MW:
22kDa
The helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. Four members of this family have been clearly implicated in tumorigenesis via their involvement in chromosomal translocations in lymphoid tumors: MYC (MIM 190080), LYL1 (MIM 151440), E2A (MIM 147141), and SCL (MIM 187040).
Purification Method:
Affinity purification
Gene ID:
4807
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with NHLH1 using NHLH1 Rabbit pAb (CAB15695) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
