The NHEJ1 Polyclonal Antibody (CAB19957) is a valuable tool for researchers studying the non-homologous end joining (NHEJ) pathway, a critical DNA repair mechanism in cells. This rabbit-derived antibody is highly specific and reactive with human samples, making it well-suited for use in Western blot applications. By targeting the NHEJ1 protein, this antibody allows for precise detection and analysis of DNA repair processes in various cell types.NHEJ1 is an essential component of the NHEJ pathway, which repairs double-strand breaks in DNA to maintain genomic integrity. Dysregulation of this pathway can lead to genetic mutations and contribute to the development of diseases such as cancer and genetic disorders.
By studying NHEJ1 and its role in DNA repair, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.With its high specificity and reliability, the NHEJ1 Polyclonal Antibody (CAB19957) is a valuable tool for research in molecular biology, genetics, and cancer biology. Its ability to target NHEJ1 protein in human samples makes it an ideal choice for studies focusing on DNA repair mechanisms and their implications for human health and disease.
Product Name:
[KO Validated] NHEJ1 Rabbit Polyclonal Antibody
SKU:
CAB19957
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human NHEJ1 (NP_079058.1).
Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.
Purification Method:
Affinity purification
Gene ID:
79840
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and NHEJ1 knockout (KO) HeLa cells, using [KO Validated] NHEJ1 Rabbit pAb (CAB19957) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
