The NGF Polyclonal Antibody (CAB14216) is specifically designed for research focusing on nerve growth factor (NGF), a key protein involved in the development and maintenance of the nervous system. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, particularly in Western blotting.NGF plays a crucial role in promoting the growth and survival of nerve cells, making it essential for studying neuronal development, regeneration, and degeneration. By binding to NGF, this antibody enables the detection and analysis of NGF in different cell types, offering valuable insights for neuroscience and neurology research.
Understanding the mechanisms of NGF signaling is vital for unraveling the complexities of neurodevelopmental disorders, neurodegenerative diseases, and nerve-related injuries. With its efficacy in detecting NGF, the NGF Polyclonal Antibody (CAB14216) serves as a valuable tool for investigating the role of NGF in various physiological and pathological processes within the nervous system.
Product Name:
NGF Rabbit Polyclonal Antibody
SKU:
CAB14216
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-241 of human NGF (NP_002497.2).
This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.
Purification Method:
Affinity purification
Gene ID:
4803
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of Mouse liver, using NGF antibody (CAB14216) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
