The NG2/CSPG4 Polyclonal Antibody (CAB22012) is a key tool for researchers studying the NG2/CSPG4 protein, a cell surface marker that plays a role in various cellular processes, including cell proliferation, migration, and invasion. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By binding to the NG2/CSPG4 protein, this antibody allows for the detection and analysis of this important molecule in different cell types, making it ideal for studies in cancer biology, stem cell research, and developmental biology.
NG2/CSPG4, also known as melanoma-associated chondroitin sulfate proteoglycan, is overexpressed in various cancer types, including melanoma, gliomas, and sarcomas. It is involved in tumor growth, invasion, and metastasis, making it a promising target for anticancer therapies. Furthermore, NG2/CSPG4 has been implicated in stem cell biology, specifically in regulating the behavior of neural stem cells and mesenchymal stem cells. Understanding the function of NG2/CSPG4 in these contexts is essential for developing novel therapeutic strategies for cancer and regenerative medicine.
Product Name:
NG2/CSPG4 Polyclonal Antibody
SKU:
CAB22012
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2).
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Purification Method:
Affinity purification
Gene ID:
1464
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using NG2/CSPG4 Rabbit pAb (CAB22012) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Negative control (NC): MCF7Exposure time: 30s.
