The NFS1 Polyclonal Antibody (CAB13385) is a crucial tool for researchers studying NFS1, a key enzyme involved in iron-sulfur cluster biogenesis and regulation of cellular metabolism. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting NFS1 in human samples, making it ideal for use in Western blot applications.NFS1 is an essential protein that plays a critical role in various cellular processes, including DNA replication, repair, and mitochondrial function. Dysregulation of NFS1 has been linked to a variety of diseases, including cancer and neurodegenerative disorders.
By targeting NFS1 with this antibody, researchers can gain valuable insights into its function and potential therapeutic implications in these pathological conditions.Overall, the NFS1 Polyclonal Antibody offers researchers a reliable tool for investigating the role of NFS1 in cellular physiology and disease pathogenesis, paving the way for the development of targeted therapies and interventions for a range of health conditions.
Product Name:
NFS1 Rabbit Polyclonal Antibody
SKU:
CAB13385
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 208-457 of human NFS1 (NP_066923.3).
Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described.
Purification Method:
Affinity purification
Gene ID:
9054
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NFS1 antibody (CAB13385) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
