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Anti-IkBAlpha Antibody (CAB1187)

SKU:
CAB1187
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
$167 - $503
Frequently bought together:

Description

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Anti-IkBAlpha Antibody (CAB1187)

The NFKBIA Polyclonal Antibody (CAB1187) is a valuable tool for researchers studying the NFKBIA protein, an important regulator in the NF-kB signaling pathway. This antibody, generated in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to NFKBIA, this antibody enables accurate detection and analysis of the protein in a variety of cell types, making it ideal for studies related to inflammation, immune response, and cancer research.NFKBIA is a key modulator of NF-kB activity, playing a crucial role in regulating gene expression in response to various stimuli.

Dysregulation of the NF-kB pathway and its components, including NFKBIA, has been implicated in numerous diseases such as cancer, inflammatory disorders, and autoimmune conditions. By understanding the function of NFKBIA, researchers can gain insights into potential therapeutic targets for these diseases, ultimately leading to the development of novel treatments that target the NF-kB signaling pathway.

Antibody Name:I?BAlpha Rabbit Polyclonal Antibody
Antibody SKU:CAB1187
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human I?BAlpha (NP_065390.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:DU145, MCF7, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human I?BAlpha (NP_065390.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MFQA AERP QEWA MEGP RDGL KKER LLDD RHDS GLDS MKDE EYEQ MVKE LQEI RLEP QEVP RGSE PWKQ QLTE DGDS FLHL AIIH EEKA LTME VIRQ VKGD LAFL NFQN NLQQ TPLH LAVI TNQP EIAE ALLG AGCD PELR DFRG NTPL HLAC EQGC LASV GVLT QSCT TPHL HSIL KATN YNGH TCLH LASI HGYL GIVE LLVS LGAD VNAQ EPCN GRTA LHLA VDLQ NPDL VSLL LKCG ADVN RVTY QGYS PYQL TWGR PSTR IQQQ LGQL TLEN LQML PESE DEES YDTE SEFT EFTE DELP YDDC VFGG QRLT L
Gene ID:4792
Uniprot:P25963
Cellular Location:Cytoplasm, Nucleus
Calculated MW:35kDa
Observed MW:39KDa
Synonyms:IKBA, MAD-3, NFKBI, IKB alpha, NFKBIA, EDAID2
Background:This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
UniProt Protein Function:Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
NCBI Summary:This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
UniProt Code:P25963
NCBI GenInfo Identifier:126682
NCBI Gene ID:4792
NCBI Accession:P25963.1
UniProt Secondary Accession:P25963,B2R8L6,
UniProt Related Accession:P25963
Molecular Weight:35,609 Da
NCBI Full Name:NF-kappa-B inhibitor alpha
NCBI Synonym Full Names:NFKB inhibitor alpha
NCBI Official Symbol:NFKBIA  
NCBI Official Synonym Symbols:IKBA; MAD-3; NFKBI  
NCBI Protein Information:NF-kappa-B inhibitor alpha
UniProt Protein Name:NF-kappa-B inhibitor alpha
UniProt Synonym Protein Names:I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3
Protein Family:NF-kappa-B inhibitor
UniProt Gene Name:NFKBIA  
UniProt Entry Name:IKBA_HUMAN
Anti-IkBAlpha Antibody (CAB1187)Western blot analysis of extracts of various cell lines, using IkBalpha antibody (CAB1187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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