The NFkB1 Polyclonal Antibody (CAB11160) is a valuable tool for researchers studying NFkB1, a key transcription factor involved in various cellular processes, including inflammation, immune response, and cell survival. This antibody, produced in rabbits, is highly specific to human NFkB1 and has been validated for use in Western blot applications.NFkB1 is a crucial regulator of immune and inflammatory responses, making it a target of interest in studies related to cancer, autoimmune diseases, and infectious diseases.
By detecting and analyzing NFkB1 protein levels in different cell types, researchers can gain insights into the role of this transcription factor in disease pathogenesis.The NFkB1 Polyclonal Antibody enables precise detection and analysis of NFkB1 in experimental settings, providing researchers with a powerful tool for investigating the molecular mechanisms underlying inflammatory and immune-related diseases. Its versatility and reliability make it an essential component of immunology and cancer research projects.
Product Name:
NFKB1 Rabbit Polyclonal Antibody
SKU:
CAB11160
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of NFKB1 (NP_001158884.1).
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. NFKB is a critical regulator of the immediate-early response to viral infection. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.
Purification Method:
Affinity purification
Gene ID:
4790
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Raji cells, using NFKB1 Rabbit pAb (CAB11160) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
