The NFIL3 Polyclonal Antibody (CAB7294) is a valuable tool for researchers studying NFIL3, a transcription factor involved in immune regulation and circadian rhythm control. This antibody, generated in rabbits, is highly specific for human NFIL3 and has been validated for use in Western blot applications. By targeting the NFIL3 protein, researchers can accurately detect and analyze NFIL3 expression in various cell types, making it ideal for studies in immunology, circadian biology, and cancer research.NFIL3, also known as E4BP4, is a critical regulator of immune cell development and function, as well as controlling the expression of genes involved in the circadian clock.
Its role in immune response modulation and circadian rhythm regulation makes it a promising target for research into diseases such as cancer, autoimmune disorders, and sleep disorders. Understanding the function of NFIL3 is essential for developing treatments that target immune function and circadian rhythm disruptions in these disease contexts.
Product Name:
NFIL3 Rabbit Polyclonal Antibody
SKU:
CAB7294
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 133-462 of human NFIL3 (NP_005375.2).
The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4783
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of SW620 cells, using NFIL3 antibody (CAB7294) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
