The NFE2L2 Polyclonal Antibody (CAB1244) is specifically designed for research involving NFE2L2, a transcription factor that plays a key role in the regulation of antioxidant response element (ARE) genes. The antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry. By binding to NFE2L2 protein, this antibody enables accurate detection and analysis in multiple cell types, making it an essential tool for studies in oxidative stress, inflammation, and cancer research.NFE2L2, also known as nuclear factor erythroid 2-related factor 2, is a crucial regulator of cellular defense mechanisms against oxidative stress and inflammation. Its involvement in the activation of antioxidant and detoxification genes makes it a central player in maintaining cellular homeostasis and protecting cells from damage.
Research on NFE2L2 is particularly important for understanding diseases associated with oxidative stress, such as neurodegenerative disorders, cardiovascular diseases, and cancer.By using the NFE2L2 Polyclonal Antibody (CAB1244), researchers can gain valuable insights into the molecular mechanisms underlying NFE2L2 activation and its impact on cellular responses to oxidative stress. This antibody is an indispensable tool for investigating the role of NFE2L2 in various physiological and pathological conditions, paving the way for the development of novel therapeutic strategies targeting NFE2L2 signaling
Product Name:
NRF2 Rabbit Polyclonal Antibody
SKU:
CAB1244
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 243-400 of human NRF2 (NP_006155.2).
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
Purification Method:
Affinity purification
Gene ID:
4780
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3,C6 cells using NRF2 Rabbit pAb (CAB1244) at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated by MG132(10 μM) at 37℃ for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 45 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
