The NFE2L2 Polyclonal Antibody (CAB0674) is a valuable tool for researchers studying NFE2L2, a transcription factor involved in the regulation of antioxidant response elements. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the NFE2L2 protein, this antibody enables detection and analysis in a variety of cell types, making it ideal for investigations in oxidative stress, inflammation, and cancer research.
NFE2L2, also known as NRF2, is a key player in the cellular defense against oxidative stress by regulating the expression of antioxidant proteins. Dysregulation of NFE2L2 has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Research into the function and regulation of NFE2L2 is essential for developing strategies to target this pathway for therapeutic interventions in oxidative stress-related diseases.
Product Name:
NRF2 Rabbit Polyclonal Antibody
SKU:
CAB0674
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 243-400 of human NRF2 (NP_006155.2).
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
Purification Method:
Affinity purification
Gene ID:
4780
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and NRF2 knockout (KO) HeLa cells using NRF2 Rabbit pAb (CAB0674) at 1:1000 dilution.wild type (WT) and Hela-NFE2L2 knockout (KO) C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s.
