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NF-kappaB p65 Transcription Factor Activity Assay

SKU:
TFAB00141
Product Type:
ELISA Kit
ELISA Type:
Transcription Factor Assay
Analytes:
NF-kappaB p65
Reactivity:
Human
Mouse
Rat
$719
Frequently bought together:

Description

system_update_altDatasheet

NF-kappaB p65 Transcription Factor Activity Assay

The NF-kappaB p65 Transcription Factor Activity Assay is a highly sensitive and specific kit designed for the accurate detection of NF-kappaB p65 transcription factor activity in various biological samples. This assay is ideal for researchers studying inflammation, immune response, and gene regulation.NF-kappaB p65 is a key transcription factor that plays a vital role in regulating the expression of genes involved in inflammatory and immune responses.

Dysregulation of NF-kappaB p65 activity has been linked to various diseases, including cancer, autoimmune disorders, and inflammatory conditions.With its high sensitivity and reliability, the NF-kappaB p65 Transcription Factor Activity Assay from Assay Genie provides researchers with a valuable tool for studying the role of NF-kappaB p65 in disease pathogenesis and developing potential therapeutic strategies.

Product Name:NF-kappaB p65 Transcription Factor Activity Assay
Product Code:TFAB00141
Target:NF-kappaB p65
Synonyms:Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Reactivity:Human, Mouse, Rat
Sample Types:Nuclear or cell lysates

The Assay Genie NF-kappaB p65 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:4.5 hours
Detection Method:Colorimetric 450 nm
Size:12 x 8-Well Microstrips
Storage:4°C for 6 months
UniProt Protein Function:NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.
UniProt Protein Details:

Protein type:DNA-binding; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function:actinin binding; chromatin binding; chromatin DNA binding; DNA binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; inflammatory response; membrane protein intracellular domain proteolysis; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; response to organic substance; response to UV-B; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway

NCBI Summary:NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:Q04206
NCBI GenInfo Identifier:62906901
NCBI Gene ID:5970
NCBI Accession:Q04206.2
UniProt Secondary Accession:Q04206,Q6GTV1, Q6SLK1,
UniProt Related Accession:Q04206
Molecular Weight:59,910 Da
NCBI Full Name:Transcription factor p65
NCBI Synonym Full Names:RELA proto-oncogene, NF-kB subunit
NCBI Official Symbol:RELA  
NCBI Official Synonym Symbols:p65; NFKB3  
NCBI Protein Information:transcription factor p65
UniProt Protein Name:Transcription factor p65
UniProt Synonym Protein Names:Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Protein Family:Proline-rich P65 protein
UniProt Gene Name:RELA  
AntibodiesELISA Kits
Anti-NF-kB p65 Mouse Monoclonal Antibody (CAB10609)NF-kappaB p65 (Phospho-Ser529) Colorimetric Cell-Based ELISA Kit
Anti-NF-kB p65 Antibody (CAB11201)[KO Validated]NF-kappaB p65 (Phospho-Ser276) Colorimetric Cell-Based ELISA Kit
Anti-NF-kB p65 Antibody (CAB11202)NF-kappaB p65 (Phospho-Ser468) Colorimetric Cell-Based ELISA Kit
Anti-NF-kB p65 Antibody (CAB11204)NF-kappaB p65 (Phospho-Thr254) Colorimetric Cell-Based ELISA Kit
Anti-NF-kB p65 Antibody (CAB16271)NF-kappaB p65 (Phospho-Thr435) Colorimetric Cell-Based ELISA Kit

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