The Neutral Ceramidase Polyclonal Antibody (CAB7985) is a valuable tool for researchers studying neutral ceramidase, an enzyme involved in the metabolism of ceramides. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to neutral ceramidase, researchers can accurately detect and analyze this important enzyme in various cell types.Neutral ceramidase plays a crucial role in the regulation of ceramide levels, which are involved in cell signaling, apoptosis, and inflammation.
Understanding the function of neutral ceramidase is essential for investigating its role in diseases such as cancer, neurodegenerative disorders, and metabolic conditions. By utilizing the Neutral Ceramidase Polyclonal Antibody, researchers can explore the mechanisms behind neutral ceramidase activity and its implications for disease pathology.
Product Name:
ASAH2 Rabbit Polyclonal Antibody
SKU:
CAB7985
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 531-780 of human ASAH2 (Q9NR71).
Cell membrane, Single-pass type II membrane protein.
Calculated MW:
86kDa
Observed MW:
100kDa
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).
Purification Method:
Affinity purification
Gene ID:
56624
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ASAH2 Rabbit pAb (CAB7985) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
