The Neurogenin-3 Polyclonal Antibody (CAB2772) is specifically designed for research involving Neurogenin-3, a transcription factor essential for the development of pancreatic islet cells and enteroendocrine cells. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications such as Western blot, immunofluorescence, and immunohistochemistry.Neurogenin-3 plays a crucial role in the differentiation and function of pancreatic endocrine cells, making it a key player in understanding diabetes and other metabolic disorders. By detecting and analyzing Neurogenin-3 protein expression in different cell types, this antibody enables researchers to further explore the mechanisms underlying pancreatic development and function.
Studies have shown that dysregulation of Neurogenin-3 expression can lead to impaired pancreatic cell differentiation and development, potentially contributing to the pathogenesis of diabetes and other metabolic diseases. Therefore, investigating the role of Neurogenin-3 using this antibody could provide valuable insights for therapeutic interventions targeting pancreatic dysfunction and related disorders.
Product Name:
NEUROG3 Rabbit Polyclonal Antibody
SKU:
CAB2772
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human NEUROG3 (NP_066279.2).
Sequence:
MTPQ PSGA PTVQ VTRE TERS FPRA SEDE VTCP TSAP PSPT RTRG NCAE AEEG GCRG APRK LRAR RGGR SRPK SELA LSKQ RRSR RKKA NDRE RNRM HNLN
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:
ngn3; Atoh5; NGN-3; Math4B; bHLHa7; NEUROG3
Positive Sample:
THP-1,Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
23kDa
Observed MW:
25kDa
The protein encoded by this gene is a basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of congenital malabsorptive diarrhea 4 (DIAR4).
Purification Method:
Affinity purification
Gene ID:
50674
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using NEUROG3 Rabbit pAb (CAB2772) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
