NETO1 Antibody (PACO58348)
- SKU:
- PACO58348
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
NETO1 Antibody (PACO58348)
The NETO1 Antibody (PAC058348) is a valuable tool for researchers studying the NETO1 protein, a key player in synaptic transmission and plasticity. This polyclonal antibody, generated in rabbits, demonstrates high specificity and sensitivity in detecting NETO1 in human samples through Western blotting techniques.NETO1, a transmembrane protein, is known to interact with ionotropic glutamate receptors and modulate their activity, thus playing a crucial role in synaptic function and plasticity. Research on NETO1 is essential for understanding the underlying mechanisms of neurological disorders such as epilepsy, schizophrenia, and Alzheimer's disease.
The NETO1 Antibody enables precise detection and analysis of NETO1 expression in various cell types, making it an indispensable tool for neuroscientists and researchers studying synaptic function and dysfunction. By elucidating the role of NETO1 in synaptic transmission, this antibody contributes to advancing our knowledge of neurological disorders and potential therapeutic targets.
| Antibody Name: | NETO1 Antibody (PACO58348) |
| Antibody SKU: | PACO58348 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Neuropilin and tolloid-like protein 1 protein (367-507AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, U87 whole cell lysate. All lanes: NETO1 antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 61, 18 kDa. Observed band size: 61 kDa. |
 | Immunofluorescence staining of HepG2 cells with PACO58348 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory (By similarity). |
| Synonyms: | Neuropilin and tolloid-like protein 1 (Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1), NETO1, BTCL1 |
| UniProt Protein Function: | NETO1: a transmembrane protein and component of the N-methyl-D-aspartate receptor (NMDAR) complex. Required to maintain the normal level of NMDAR subunits at synapses. The NMDAR complex is a major excitatory ligand-gated ion channel in the central nervous system and a major mediator of synaptic plasticity. Important in the development and/or maintenance of neuronal circuitry. Neto1-deficient mice have a dramatic decrease in the number of NMDA receptors at synapses, impairing learning and synaptic plasticity. Three isoforms of the human protein are produced by alternative splicing. Isoforms 1 and 2 are retina-specific whereas isoform 3 is found in retina as well as at lower levels in adult and fetal brain.Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 18q22.2Cellular Component: postsynaptic membrane; kainate selective glutamate receptor complex; postsynaptic density; extracellular region; excitatory synapse; cell junctionMolecular Function: ionotropic glutamate receptor bindingBiological Process: regulation of long-term neuronal synaptic plasticity; visual learning; memory |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a predicted transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. A similar gene in mice encodes a protein that plays a critical role in spatial learning and memory by regulating the function of synaptic N-methyl-D-aspartic acid receptor complexes in the hippocampus. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
| UniProt Code: | Q8TDF5 |
| NCBI GenInfo Identifier: | 319918840 |
| NCBI Gene ID: | 81832 |
| NCBI Accession: | NP_001188394.1 |
| UniProt Secondary Accession: | Q8TDF5,Q86W85, Q8ND78, Q8TDF4 |
| UniProt Related Accession: | Q8TDF5 |
| Molecular Weight: | 60,060 Da |
| NCBI Full Name: | neuropilin and tolloid-like protein 1 isoform 3 |
| NCBI Synonym Full Names: | neuropilin (NRP) and tolloid (TLL)-like 1 |
| NCBI Official Symbol: | NETO1Â Â |
| NCBI Official Synonym Symbols: | BCTL1; BTCL1Â Â |
| NCBI Protein Information: | neuropilin and tolloid-like protein 1; brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 |
| UniProt Protein Name: | Neuropilin and tolloid-like protein 1 |
| UniProt Synonym Protein Names: | Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 |
| Protein Family: | |
| UniProt Gene Name: | NETO1Â Â |
| UniProt Entry Name: | NETO1_HUMAN |
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