The Nephrocystin-1 Polyclonal Antibody (CAB6674) is a valuable tool for researchers studying nephrocystin-1, a protein involved in ciliogenesis and cilia function. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it an ideal choice for immunofluorescence and immunohistochemistry applications.Nephrocystin-1 is a key player in the development and maintenance of primary cilia, cellular appendages with crucial roles in signaling pathways and cell cycle regulation.
Mutations in nephrocystin-1 have been linked to ciliopathies, a group of genetic disorders affecting cilia structure and function. By using the Nephrocystin-1 Polyclonal Antibody, researchers can investigate the expression and localization of nephrocystin-1 in various cell types and tissues, providing insights into ciliopathy pathogenesis and potential therapeutic targets.
Product Name:
NPHP1 Rabbit Polyclonal Antibody
SKU:
CAB6674
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 463-732 of human NPHP1 (NP_997064.2).
This gene encodes a protein with src homology domain 3 (SH3) patterns. This protein interacts with Crk-associated substrate, and it appears to function in the control of cell division, as well as in cell-cell and cell-matrix adhesion signaling, likely as part of a multifunctional complex localized in actin- and microtubule-based structures. Mutations in this gene cause familial juvenile nephronophthisis type 1, a kidney disorder involving both tubules and glomeruli. Defects in this gene are also associated with Senior-Loken syndrome type 1, also referred to as juvenile nephronophthisis with Leber amaurosis, which is characterized by kidney and eye disease, and with Joubert syndrome type 4, which is characterized by cerebellar ataxia, oculomotor apraxia, psychomotor delay and neonatal breathing abnormalities, sometimes including retinal dystrophy and renal disease. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4867
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse pancreas, using NPHP1 Rabbit pAb (CAB6674) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
