The Nephrin Polyclonal Antibody (CAB3048) is a valuable tool for researchers studying nephrin, an important protein involved in the function of the kidney's filtration barrier. This antibody, developed in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the nephrin protein, researchers can accurately detect and analyze its presence in various cell types, making it ideal for investigations in nephrology and kidney-related disorders.
Nephrin is a key component of the podocyte slit diaphragm, playing a crucial role in maintaining the integrity of the kidney filtration system. Dysregulation of nephrin expression has been associated with various kidney diseases, including nephrotic syndrome and diabetic nephropathy. By studying the function and regulation of nephrin using this antibody, researchers can gain valuable insights into the pathogenesis of these conditions and potentially identify new therapeutic targets for treatment.
Product Name:
NPHS1 Rabbit Polyclonal Antibody
SKU:
CAB3048
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1019-1241 of human NPHS1 (NP_004637.1).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
135kDa
Observed MW:
130kDa/180kDa
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.
Purification Method:
Affinity purification
Gene ID:
4868
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse kidney, using NPHS1 Rabbit pAb (CAB3048) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
