The NELFE Polyclonal Antibody (CAB7006) is a valuable tool for researchers studying NELFE, a protein involved in RNA processing and transcriptional regulation. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By binding to NELFE, this antibody enables the detection and analysis of NELFE in various cell types, making it ideal for studies in molecular biology and gene expression research.NELFE is a key player in the regulation of RNA polymerase II transcription and RNA processing, making it essential for proper gene expression and cellular function.
Dysregulation of NELFE has been linked to various diseases, including cancer and neurodevelopmental disorders, highlighting its importance in biological processes. Investigating the role of NELFE can provide valuable insight into disease mechanisms and potential therapeutic targets in these areas.Overall, the NELFE Polyclonal Antibody (CAB7006) is a reliable tool for researchers interested in studying the functions of NELFE and its implications in various biological processes and diseases. Its high specificity and sensitivity make it a valuable asset for experiments involving gene expression and RNA regulation.
Product Name:
NELFE Rabbit Polyclonal Antibody
SKU:
CAB7006
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3).
The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
7936
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NELFE antibody (CAB7006) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
