The NELFE Polyclonal Antibody (CAB3249) is a high-quality antibody designed for research involving NELFE, a key protein involved in transcriptional regulation. Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for use in various experimental applications such as Western blotting. By targeting the NELFE protein, this antibody enables precise detection and analysis in different cell types, making it a valuable tool for research in molecular biology, gene expression, and transcriptional control mechanisms.NELFE, also known as Negative elongation factor E, plays a critical role in regulating RNA polymerase II transcription elongation and gene expression.
Understanding the function of NELFE is essential for unraveling the complex mechanisms of transcriptional regulation and gene expression, making this antibody a valuable asset for researchers studying molecular processes involved in various physiological and pathological conditions.Overall, the NELFE Polyclonal Antibody (CAB3249) is a reliable tool for researchers looking to investigate the role of NELFE in gene expression and transcriptional control, providing valuable insights into the underlying molecular mechanisms that govern these processes.
Product Name:
NELFE Rabbit Polyclonal Antibody
SKU:
CAB3249
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3).
The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
7936
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using NELFE Rabbit pAb (CAB3249) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
