The NEK4 Polyclonal Antibody (CAB16433) is a valuable tool for researchers studying NEK4, a protein kinase involved in cell division and cell cycle regulation. This antibody, generated in rabbits, shows high specificity and sensitivity towards NEK4 in human samples, making it ideal for Western blot applications. By binding to NEK4, this antibody enables the accurate detection and analysis of NEK4 expression in various cell types, facilitating research in areas such as cell biology, cancer, and developmental biology.NEK4, a member of the NIMA-related kinase family, plays a crucial role in mitotic processes and is implicated in cellular functions such as centrosome duplication and spindle organization.
Dysregulation of NEK4 has been linked to abnormal cell division and cancer development, highlighting its importance as a potential therapeutic target. By studying NEK4 expression and activity, researchers can gain insights into its function and potential implications in disease pathology, paving the way for the development of novel treatment strategies.
Product Name:
NEK4 Rabbit Polyclonal Antibody
SKU:
CAB16433
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK4 (NP_003148.2).
The protein encoded by this gene is a serine/threonine protein kinase required for normal entry into replicative senescence. The encoded protein also is involved in cell cycle arrest in response to double-stranded DNA damage. Finally, this protein plays a role in maintaining cilium integrity, and defects in this gene have been associated with ciliopathies.
Purification Method:
Affinity purification
Gene ID:
6787
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NEK4 Rabbit pAb (CAB16433) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
