The NEK3 Polyclonal Antibody (CAB6665) is a valuable tool for researchers studying NEK3, a member of the NIMA-related kinase family which plays a key role in cell cycle regulation and signaling pathways. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By targeting the NEK3 protein, this antibody allows for precise detection and analysis in various cell types, making it an essential component for studies in cell biology and cancer research.NEK3 is involved in crucial cellular processes such as cell division, proliferation, and DNA damage response, making it a promising target for investigating diseases like cancer and genetic disorders.
Understanding the functions of NEK3 is essential for unraveling its role in disease pathology and developing targeted therapies for conditions related to cell cycle dysregulation. This NEK3 Polyclonal Antibody offers researchers a reliable tool for exploring the intricate mechanisms of NEK3 and its implications in various biological processes.
Product Name:
NEK3 Rabbit Polyclonal Antibody
SKU:
CAB6665
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 267-506 of human NEK3 (NP_689933.1).
This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Two functional alleles for this gene have been identified in humans. The reference genome assembly (GRCh38) represents a functional allele that is associated with the inclusion of an additional coding exon in protein-coding transcripts, compared to an alternate functional allele that lacks the exon.
Purification Method:
Affinity purification
Gene ID:
4752
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NEK3 antibody (CAB6665) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
