The NEK2 Polyclonal Antibody (CAB5355) is a vital tool for researchers studying the NEK2 protein, a key regulator of cell division and a potential target for cancer therapy. This antibody, generated using rabbit immunization, exhibits high specificity for human samples and is validated for use in Western blot applications. It specifically binds to the NEK2 protein, allowing for accurate detection and analysis in various cell types.NEK2 is a member of the NIMA-related kinase family and plays a crucial role in regulating mitotic processes, making it a promising target for cancer research and drug development. Dysregulation of NEK2 has been implicated in various types of cancer, highlighting its potential as a therapeutic target.
By studying the function of NEK2 using this antibody, researchers can gain valuable insights into the mechanisms of cancer progression and identify potential strategies for targeted therapy.Overall, the NEK2 Polyclonal Antibody (CAB5355) is an essential tool for investigating the role of NEK2 in cancer biology and advancing our understanding of cell division processes. Its high reactivity and specificity make it an invaluable resource for researchers looking to uncover the potential of NEK2 as a therapeutic target in cancer treatment.
Product Name:
NEK2 Rabbit Polyclonal Antibody
SKU:
CAB5355
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human NEK2 (NP_001191112.1).
Chromosome, Cytoplasm, Nucleus, centromere, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, nucleolus, spindle pole.
Calculated MW:
52kDa
Observed MW:
52kDa
This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.
Purification Method:
Affinity purification
Gene ID:
4751
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using NEK2 antibody (CAB5355) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
