The NEK2 Polyclonal Antibody (CAB13334) is a vital tool for research involving NEK2, a crucial protein kinase involved in cell cycle regulation and spindle assembly during mitosis. This antibody, generated in rabbits, exhibits strong reactivity with human samples and is optimized for use in Western blot applications. By binding specifically to NEK2, this antibody enables accurate detection and analysis of NEK2 expression in various cell types, making it an invaluable resource for studies in cell biology, cancer research, and drug development.
NEK2 is a key player in cell division, ensuring proper chromosome segregation and cell proliferation. Dysregulation of NEK2 has been linked to tumor formation and progression, highlighting its potential as a therapeutic target in cancer treatment. By further investigating the function of NEK2, researchers can uncover new insights into cell cycle control mechanisms and potentially develop targeted therapies for cancer and other proliferative disorders.
Product Name:
NEK2 Rabbit Polyclonal Antibody
SKU:
CAB13334
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 345-445 of human NEK2 (NP_002488.1).
Chromosome, Cytoplasm, Nucleus, centromere, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, nucleolus, spindle pole.
Calculated MW:
52kDa
Observed MW:
52kDa
This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.
Purification Method:
Affinity purification
Gene ID:
4751
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using NEK2 Rabbit pAb (CAB13334) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
