The NEK10 Polyclonal Antibody (PAC062491) is a valuable tool for researchers studying NEK10, a protein kinase involved in regulating cell cycle progression and DNA damage response. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to NEK10, this antibody allows for accurate detection and analysis of the protein in a variety of cell types, making it an essential tool for studies in cancer biology and cell signaling pathways.
NEK10 is known to play a crucial role in maintaining genomic stability and preventing the development of genetic abnormalities that can lead to cancer. Its involvement in cell cycle regulation makes it a promising target for research into therapeutics that target cancer cell proliferation. By studying the function of NEK10, researchers can gain valuable insights into the mechanisms underlying cancer development and potentially identify new therapeutic strategies for treating the disease.
Antibody Name:
NEK10 Antibody (PACO62491)
Antibody SKU:
PACO62491
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human Serine/threonine-protein kinase Nek10 protein (1-104AA)
IHC image of PACO62491 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO62491 diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
protein kinase complex, protein kinase activity, positive regulation of MAP kinase activity, positive regulation of protein autophosphorylation, protein phosphorylation, regulation of cell cycle G2/M phase transition, regulation of ERK1 and ERK2 cascade
Synonyms:
Serine/threonine-protein kinase Nek10 (EC 2.7.11.1) (Never in mitosis A-related kinase 10) (NimA-related protein kinase 10), NEK10
UniProt Protein Function:
NEK10: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. 7 isoforms of the human protein are produced by alternative splicing.Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; NEK familyChromosomal Location of Human Ortholog: 3p24.1Cellular Component: centrosome; cytoplasm; nucleusMolecular Function: protein kinase activityBiological Process: chromosome segregation; microtubule-based process; positive regulation of MAP kinase activity; positive regulation of protein amino acid autophosphorylation; protein amino acid phosphorylation
